The methanol extract of Actaea acuminata roots has been previously reported to exhibit significant antianxiety, anticonvulsant and antidepressant activities. In the present study fractionation of crude bioactive methanol extract was carried out using different solvents employing standard procedure. Various fractions were evaluated for antianxiety, anticonvulsant and antidepressant activities using elevated plus maze model (EPM), maximal electroshock-induced convulsions model (MES) and forced / despair swim test (FST) respectively. Successive partitioning of bioactive methanol extract was done with ethyl acetate and 1-butanol. The ethyl acetate fraction (EAF; 100 mg/kg), 1-butanol fraction (BF; 25 mg/kg) and remaining methanol extract (RME; 70 mg/kg) were subjected to neuropharmacological activities.
The EAF significantly enhanced entries and average time spent in open arms; significantly decreased the duration of MES-induced tonic extension phase and significantly decreased the duration of immobility time of rats in comparison to control. BF and RME did not exhibit any neuropharmacological activity. Qualitative chemical tests confirmed the presence of alkaloids and polyphenols in EAF. It is finally concluded that alkaloids and/or polyphenols are bioactive constituents of A. acuminata which are responsible for neuropharmacological activities.
Deepak Kumar and Suresh Kumar
Actaea acuminata, Alkaloids, Flavonoids, Himalayan Baneberry.
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