Chitkara Open Access Journals - Blog

Sperm Hyperactivation and Capacitation Induced By Light Stimuli in Cryopreserved Semen

In mammals, such as rabbits, there are some factors involved in a possible fertilization, from complex changes in the membrane of the sperm to obstruction or non-existent of vas deferens, which creates problems in the number and quality of sperm. In this work, we report effects of rabbit sperm motility and capacitation of cryopreserved semen samples under light stimuli. The sperm velocities were correlated with the percentage of capacitated and non-capacitated sperm seen with a fluorescent dye. Consequently, we analyzed the specific correlations between irradiation times, supplied energy and fertility parameters.


  • V. Soto, M.A. Fuentes, G. Navidad, R.N. Meza, A.G. Salazar and D. Osorio-GonzalezLaboratorio de Biofísica Molecular de la Facultad de Ciencias. Universidad Autónoma del Estado de México
  • L.A. MandujanoLaboratorio de Diseño y Modelado Biofísico Molecular de la Universidad Mexiquense, S. C.
  • C. FernandezClinica Fertilidad “Biología de la Reproducción” del Hospital Materno Perinatal “Mónica Pretelini Sáenz” Adscripción
  • C. Garcia and Y.E. Felipe-PerezLaboratorio de Biología de la Reproducción de la Facultad de Medicina Veterinaria y Zootecnia. Universidad Autónoma del Estado de México


Sperm capacitation, Fertility, light stimuli



Dohle G.R., Diemer T., Giwercman A., Jungwirth A., Kopa Z., Krausz C. (2010). Guía clínica sobre la infertilidad masculina. European Association of Urology

Tapia S. P. (2012). Una visión actual de la infertilidad masculina. Rev. Mex. Reprod. 4(3), 103–109

Corral B. M. I., Rigau T., Rivera M., Rodrıguez J. E., Rigau J. (2005). Effect of 655-nm diode laser on dog sperm motility. Lasers in Medical Science 20, 28–34

Holt WV, Watson P, Curry M, Holt C. (1994). Reproducibility of computeraided semen analysis: comparison of five different systems used in a practical workshop. Fertil Steril 62, 1277–1282

Fernandez GHC, de Carvalho PdTC, Serra AJ, Crespilho AM, Peron JPS, Rossato C, et al. (2015). The Effect of Low-Level Laser Irradiation on Sperm Motility, and Integrity of the Plasma Membrane and Acrosome in Cryopreserved Bovine Sperm. PLoS ONE 10(3): e0121487. doi:10.1371/journal.pone.0121487

Lubart R, Levinshal T, Cohen N, Friedmann H y Breitbart H. (1996). Changes in Calcium Transport in Mammalian Sperm Mitochondria and Plasma Membrane due to 633 nm and 780 nm Irradiation. Laser in der Medizin / Laser in Medicine, 449–453.

Oehninger S. (2010). Strategies for the infertile man. Sem Reprod Med; 19(3), 231–7.

Flaherty C. O. (2015). Redox regulation of mammalian sperm capacitation. Asian Journal of Andrology. 17, 583–590.

Roldan-Schuth, E. R. S. (2010). Biología de la reproducción de mamíferos en peligro de extinción. REDVET. Revista electrónica de Veterinaria 1695-7504. Volumen 11 Número 07

Hafez Y. M. (2015). Assisted reproductive technologies in farm Animals. ICMALPS, Alexandria University, Egypt